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The isolation of tetrangomycin from terrestrial Streptomyces sp. CAH29: evaluation of antioxidant, anticancer, and anti-MRSA activity

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dc.contributor.author Özakin, S.
dc.contributor.author Davis, R.W.
dc.contributor.author Umile, T.P.
dc.contributor.author Pirinccioglu, N.
dc.contributor.author Kizil, M.
dc.contributor.author Celik, G.
dc.contributor.author Sen, A.
dc.contributor.author Minbiole, K.P.C.
dc.contributor.author İnce, E.
dc.date.accessioned 2019-08-16T13:00:06Z
dc.date.available 2019-08-16T13:00:06Z
dc.date.issued 2016
dc.identifier.issn 10542523 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/9350
dc.description.abstract A rhizosphere isolate Streptomyces sp. CAH29 was found to possess potent antibacterial and antifungal activity against a variety of test organisms. Based on 16S ribosomal ribonucleic acid sequence homology studies, this strain was found to be similar to Streptomyces stramineus (gene sequence similarity 99 %). The major bioactive metabolite produced by Streptomyces sp. CAH29 isolate was extracted, purified andidentified by nuclear magnetic resonance as tetrangomycin. This known anthraquinone-exhibited antimicrobial activity against Staphylococcus aureus, Streptococcus pyogenes, methicillin resistant Staphylococcus aureus and Candida albicans with inhibition zones of 14, 10, 12 and 8 mm, respectively. Docking results demonstrate that tetrangomycin has a similar mode of action and a comparable docking score to bind to the dehydrosqualene synthase (CrtM) enzyme of methicillin resistant Staphylococcus aureus compared to the current inhibitor. Hence, this suggests that tetrangomycin has a potential to be used as an anti-methicillin resistant Staphylococcus aureus agent. Tetrangomycin also showed moderate free radical scavenging activity with 1,1-diphenyl-2-picryl-hydrazil. Tetrangomycin apparently decreased all of the studied cytokine (pro-inflammatory: interleukin 1B, interleukin 2, tumor necrosis factor and interleukin L6 and anti-inflammatory: interleukin 10) expression levels at IC50 concentrations in A459 (adenocarcinomic human alveolar basal epithelial) and LNCAP (human prostate adenocarcinoma) cell lines. In addition, it reduced Caspase 8 and 3 mRNA levels in LNCAP and A549 cells. This study describes for the first time novel in vitro immunosuppressive function of tetrangomycin by reducing the transcription of cytokine genes. © 2016, Springer Science+Business Media New York.
dc.language.iso English
dc.publisher Birkhauser Boston
dc.relation.isversionof 10.1007/s00044-016-1708-6
dc.subject Angucylines
dc.subject Anti-MRSA
dc.subject Antimicrobial activity
dc.subject Cytotoxic activity
dc.subject Immunosuppressive activity
dc.subject Streptomyces
dc.subject Tetrangomycin
dc.subject antiinfective agent
dc.subject antineoplastic agent
dc.subject antioxidant
dc.subject arginine
dc.subject bacterial enzyme
dc.subject bacterial extract
dc.subject caspase 8
dc.subject cyclin D1
dc.subject cyclin D2
dc.subject glutamine
dc.subject interleukin 10
dc.subject interleukin 1beta
dc.subject interleukin 2
dc.subject interleukin 6
dc.subject leucine
dc.subject messenger RNA
dc.subject mitogen activated protein kinase
dc.subject phosphatidylinositol 3,4,5 trisphosphate 3 phosphatase
dc.subject protein Bax
dc.subject protein bcl 2
dc.subject protein p53
dc.subject Raf protein
dc.subject RNA 16S
dc.subject stress activated protein kinase
dc.subject synthetase
dc.subject tetrangomycin
dc.subject tumor necrosis factor
dc.subject tyrosine
dc.subject unclassified drug
dc.subject unindexed drug
dc.subject valine
dc.subject A549 cell line
dc.subject antibacterial activity
dc.subject antifungal activity
dc.subject antineoplastic activity
dc.subject antioxidant activity
dc.subject Article
dc.subject biological activity
dc.subject Candida albicans
dc.subject concentration response
dc.subject controlled study
dc.subject cytotoxicity
dc.subject drug isolation
dc.subject drug structure
dc.subject enzyme binding
dc.subject epithelium basal cell
dc.subject fermentation medium
dc.subject human
dc.subject human cell
dc.subject IC50
dc.subject LNCaP cell line
dc.subject lung adenocarcinoma
dc.subject lung alveolus epithelium
dc.subject methicillin resistant Staphylococcus aureus
dc.subject molecular docking
dc.subject nonhuman
dc.subject nuclear magnetic resonance
dc.subject prostate adenocarcinoma
dc.subject prostate cancer cell line
dc.subject protein expression
dc.subject rhizosphere bacterium
dc.subject RNA sequence
dc.subject sequence homology
dc.subject Staphylococcus aureus
dc.subject Streptococcus pyogenes
dc.subject Streptomyces sp cah29
dc.subject terrestrial species
dc.title The isolation of tetrangomycin from terrestrial Streptomyces sp. CAH29: evaluation of antioxidant, anticancer, and anti-MRSA activity
dc.type Article
dc.relation.journal Medicinal Chemistry Research
dc.identifier.volume 25
dc.identifier.issue 12
dc.identifier.startpage 2872
dc.identifier.endpage 2881
dc.identifier.index Scopus


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