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Chondrocyte proliferation, viability and differentiation is declined following administration of methylphenidate utilized for the treatment of attention-deficit/hyperactivity disorder

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dc.contributor.author Gumustas, F.
dc.contributor.author Yilmaz, I.
dc.contributor.author Sirin, D.Y.
dc.contributor.author Gumustas, S.A.
dc.contributor.author Batmaz, A.G.
dc.contributor.author Isyar, M.
dc.contributor.author Akkaya, Semih
dc.contributor.author Mahirogullari, M.
dc.date.accessioned 2019-08-16T12:57:19Z
dc.date.available 2019-08-16T12:57:19Z
dc.date.issued 2017
dc.identifier.issn 09603271 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/8949
dc.description.abstract Purpose: Methylphenidate (MPH) derivative drugs are used because of psychostimulants effects on attention-deficit hyperactivity disorder in children and adults. As far as we know, toxic or anti-proliferative effects of MPH against cartilage tissue were not studied in the literature. The present study was carried out to investigate the possible effects of MPH on the proliferation, viability and differentiation of primary human chondrocytes, in vitro. Methods: Monolayer primary chondrocyte cultures were prepared using osteochondral tissue obtained from patients who underwent a total knee prosthesis operation. Stock solution of MPH was prepared and aliquots having 1-1000 μM concentrations of the drug was composed. These solutions were applied to the wells containing cultured chondrocyte samples within the well plates. Control groups were composed of pure chondrocyte culture and no solution was added into them. All groups were evaluated at 24, 48 and 72 h in order to determine the possible negative effects of the drug on the chondrocytes. The data were evaluated by Tukey's honestly significantly different test following analysis of variance. Results: In the group where MPH was applied, it was found that viability, proliferation and stage-specific embryonic antigen-1 protein expression were decreased in comparison to the control group. Conclusions: It was emphasized that clinicians should not disregard the fact that this drug might suppress chondrocyte cell proliferation and chondrogenic differentiation. © SAGE Publications.
dc.language.iso English
dc.publisher SAGE Publications Ltd
dc.relation.isversionof 10.1177/0960327116678294
dc.rights info:eu-repo/semantics/closedAccess
dc.subject ADHD
dc.subject chondrotoxicity
dc.subject methylphenidate
dc.subject proliferation
dc.subject SSEA-1
dc.subject stage specific embryo antigen 1
dc.subject Article
dc.subject attention deficit disorder
dc.subject cartilage
dc.subject cell differentiation
dc.subject cell proliferation
dc.subject cell viability
dc.subject chondrocyte
dc.subject comparative study
dc.subject concentration response
dc.subject controlled study
dc.subject drug cytotoxicity
dc.subject drug effect
dc.subject human
dc.subject human cell
dc.subject human tissue
dc.subject in vitro study
dc.subject mesenchymal stem cell
dc.subject monolayer culture
dc.subject primary culture
dc.subject priority journal
dc.subject protein expression
dc.subject surgical patient
dc.subject total knee arthroplasty
dc.subject cell culture
dc.subject Attention Deficit Disorder with Hyperactivity
dc.subject Cell Differentiation
dc.subject Cell Proliferation
dc.subject Cells, Cultured
dc.subject Chondrocytes
dc.subject Humans
dc.subject Methylphenidate
dc.title Chondrocyte proliferation, viability and differentiation is declined following administration of methylphenidate utilized for the treatment of attention-deficit/hyperactivity disorder
dc.type Article
dc.relation.journal Human and Experimental Toxicology
dc.identifier.volume 36
dc.identifier.issue 9
dc.identifier.startpage 981
dc.identifier.endpage 992
dc.relation.publicationCategory Uluslararası Hakemli Dergi
dc.identifier.index Scopus
dc.identifier.index WOS

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