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Aqueous size exclusion chromatography in semimicro and micro-columns by newly synthesized monodisperse macroporous hydrophilic beads as a stationary phase

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dc.contributor.author Gölgelioĝlu, Ç.
dc.contributor.author Bayraktar, A.
dc.contributor.author Çelebi, B.
dc.contributor.author Uğuzdoğan, Erdal
dc.contributor.author Tuncel, A.
dc.date.accessioned 2019-08-16T12:44:59Z
dc.date.available 2019-08-16T12:44:59Z
dc.date.issued 2012
dc.identifier.issn 00219673 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/8686
dc.description.abstract A new class of monodisperse macroporous beads in the hydrophilic form were synthesized by seeded microsuspension copolymerization of two acrylic crosslinking agents, glycerol dimethacrylate (GDMA) and glycerol-1,3-diglycerolate diacrylate (GDGDA). The monodisperse porous poly(glycerol dimethacrylate-co-glycerol-1,3-diglycerolate diacrylate), poly(GDMA-co-GDGDA) beads were highly hydrophilic in nature due to hydroxyl functionality resulting from both crosslinking agents. The beads with different particle sizes between 4.5 and 6.7μm and with different porous properties were obtained by changing the seed latex to monomer ratio. The bead size decreased, the average pore size increased and the specific surface area decreased with increasing seed latex to monomer ratio. Poly(GDMA-co-GDGDA) beads were slurry packed in microbore and semimicro-HPLC columns and successfully used as a stationary phase in aqueous size exclusion chromatography (SEC) mode in a micro-liquid chromatography system. The aqueous SEC runs were performed by using dextran standards in the molecular weight range of 1000-670,000Da. SEC calibration curves exhibiting linearity in a wider range of molecular weight were obtained with the semi-micro and micro-HPLC columns packed with the poly(GDMA-co-GDGDA) beads synthesized with the seed latex to monomer ratios of 0.038 and 0.058g/mL. The dextran standards could be eluted in an analysis time shorter than 2min using micro or semi-micro columns packed with poly(GDMA-co-GDGDA) beads as stationary medium. These packings are suitable for molecular weight determination between 5×10 3 and 5×10 5Da in the aqueous medium by using mobile phase flow rates in the range of 25-250μL/min. The average molecular weight determinations of different water soluble polymers, an ionic polymer, poly(2-dimethylaminoethyl methacrylate), a zwitterionic polymer, poly([2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide), and a non-ionic polymer, poly(vinyl alcohol) were performed on a semimicro-column packed with the poly(GDMA-co-GDGDA) beads. Satisfactory results were obtained in the molecular weight determination of hydrophilic polymers by aqueous SEC. © 2011 Elsevier B.V.
dc.language.iso English
dc.relation.isversionof 10.1016/j.chroma.2011.12.043
dc.rights info:eu-repo/semantics/closedAccess
dc.subject Aqueous size exclusion chromatography
dc.subject Hydrogel beads
dc.subject Micro-liquid chromatography
dc.subject Microbore columns
dc.subject Monodisperse-porous beads
dc.subject Seeded polymerization
dc.subject Ammonium hydroxide
dc.subject Analysis time
dc.subject Aqueous medium
dc.subject Average molecular weight
dc.subject Bead size
dc.subject Calibration curves
dc.subject Cross linking agents
dc.subject Diacrylates
dc.subject Dimethacrylates
dc.subject Hydrophilic polymers
dc.subject Hydroxyl functionality
dc.subject Ionic polymer
dc.subject Macroporous
dc.subject Macroporous beads
dc.subject Micro-HPLC
dc.subject Microsuspension
dc.subject Mobile phase flow rate
dc.subject Molecular weight determination
dc.subject Monodisperse
dc.subject Monomer ratio
dc.subject Nonionic polymers
dc.subject Porous properties
dc.subject Seed latexes
dc.subject Stationary phase
dc.subject Watersoluble polymers
dc.subject Zwitterionic polymers
dc.subject Ammonium compounds
dc.subject Copolymerization
dc.subject Dextran
dc.subject Glycerol
dc.subject Hydrogels
dc.subject Hydrophilicity
dc.subject Latexes
dc.subject Liquid chromatography
dc.subject Liquids
dc.subject Molecular weight
dc.subject Monomers
dc.subject Polymers
dc.subject Seed
dc.subject Size exclusion chromatography
dc.subject acrylic acid derivative
dc.subject copolymer
dc.subject dextran
dc.subject glycerol 1,3 diglycerolate diacrylate
dc.subject glycerol dimethacrylate
dc.subject glycerol dimethacrylate co glycerol 1,3 diglycerolate diacrylate
dc.subject hydroxyl group
dc.subject latex
dc.subject methacrylic acid derivative
dc.subject monomer
dc.subject poly [2 (methacryloyloxy)ethyl]dimethyl (3 sulfopropyl)ammonium hydroxide
dc.subject poly[2 (dimethylamino)ethyl methacrylate]
dc.subject polymer
dc.subject polyvinyl alcohol
dc.subject unclassified drug
dc.subject aqueous solution
dc.subject article
dc.subject controlled study
dc.subject cross linking
dc.subject flow rate
dc.subject gel permeation chromatography
dc.subject high performance liquid chromatography
dc.subject hydrophilicity
dc.subject liquid chromatography
dc.subject micro liquid chromatography
dc.subject molecular weight
dc.subject particle size
dc.subject polymerization
dc.subject porosity
dc.subject priority journal
dc.subject suspension
dc.subject synthesis
dc.subject Acrylates
dc.subject Calibration
dc.subject Chromatography, Gel
dc.subject Hydrophobic and Hydrophilic Interactions
dc.subject Methacrylates
dc.subject Microscopy, Electron, Scanning
dc.subject Microspheres
dc.subject Molecular Weight
dc.subject Particle Size
dc.subject Porosity
dc.title Aqueous size exclusion chromatography in semimicro and micro-columns by newly synthesized monodisperse macroporous hydrophilic beads as a stationary phase
dc.type Article
dc.relation.journal Journal of Chromatography A
dc.identifier.volume 1224
dc.identifier.startpage 43
dc.identifier.endpage 50
dc.relation.publicationCategory Uluslararası Hakemli Dergi
dc.identifier.index Scopus
dc.identifier.index WOS
dc.identifier.index PubMed

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