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Detection of deleted in malignant brain tumors 1 and runt-related transcription factor 3 gene expressions in bladder carcinoma

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dc.contributor.author Dodurga, Yavuz
dc.contributor.author Avci, C.B.
dc.contributor.author Satıroğlu-Tufan, Naciye lale
dc.contributor.author Tataroglu, C.
dc.contributor.author Kesen, Z.
dc.contributor.author Dogan, Z.O.
dc.contributor.author Yilmaz, S.
dc.contributor.author Gündüz, C.
dc.date.accessioned 2019-08-16T12:44:12Z
dc.date.available 2019-08-16T12:44:12Z
dc.date.issued 2012
dc.identifier.issn 03014851 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/8646
dc.description.abstract Bladder cancer is the fifth most commonly diagnosed cancer in the United States, where the majority of tumors are transitional cell carcinoma. Deleted in malignant brain tumors 1 (DMBT1) gene is located at chromosome 10q25.3-q26.1. DMBT1 gene expression has yet to be investigated in patients with bladder cancer. Runtrelated transcription factor 3 (RUNX3) is a candidate tumor suppressor gene which is localized on the chromosome 1p36. RUNX3 gene expression in bladder carcinogenesis is particularly unknown. We aimed to evaluate DMBT1 and RUNX3 gene expression profiles in bladder cancer and how their expressions could be related to carcinogenesis in the bladder and their correlation with clinicopathological parameters. Fifty-six paraffin embedded specimens of transitional cell carcinoma of the urinary bladder were used. Total RNA was extracted from bladder specimens and cDNA was synthesized. The quantification of DMBT1 and RUNX3 mRNAs were succeeded according to the manufacturers' instructions by using RT-PCR. DMBT1 and RUNX3 gene expressions were identified in 100% of bladder carcinoma samples. No significant association was found in these genes expression levels when compared to sex and age. RUNX3 gene expression was decreased nonsignificantly in high-grade tumors. When DMBT1 gene expression was compared to tumor grades, a significant decrease was detected between grade I and III (P = 0.028). Disruption of expression in relation to tumor suppressors like DMBT1 and RUNX3 genes was associated with bladder cancer. Furthermore, detailed studies including these genes should be performed in protein levels and used more patient specimens in a large scale study. © Springer Science+Business Media B.V. 2011.
dc.language.iso English
dc.relation.isversionof 10.1007/s11033-011-1261-9
dc.rights info:eu-repo/semantics/closedAccess
dc.subject Bladder cancer
dc.subject DMBT1
dc.subject Paraffin tissue
dc.subject RUNX3
dc.subject complementary DNA
dc.subject messenger RNA
dc.subject paraffin
dc.subject transcription factor RUNX3
dc.subject cell surface receptor
dc.subject DMBT1 protein, human
dc.subject Runx3 protein, human
dc.subject aged
dc.subject article
dc.subject bladder carcinogenesis
dc.subject bladder carcinoma
dc.subject cancer grading
dc.subject cancer localization
dc.subject cancer prognosis
dc.subject clinical feature
dc.subject deleted in malignant brain tumors 1 gene
dc.subject disease association
dc.subject DNA sequence
dc.subject DNA synthesis
dc.subject down regulation
dc.subject female
dc.subject gene expression profiling
dc.subject gene identification
dc.subject genetic association
dc.subject human
dc.subject human tissue
dc.subject male
dc.subject molecular pathology
dc.subject nucleotide sequence
dc.subject oncogene
dc.subject reverse transcription polymerase chain reaction
dc.subject RNA extraction
dc.subject runt related transcription factor 3 gene
dc.subject transitional cell carcinoma
dc.subject bladder tumor
dc.subject gene expression regulation
dc.subject genetics
dc.subject metabolism
dc.subject middle aged
dc.subject pathology
dc.subject Aged
dc.subject Core Binding Factor Alpha 3 Subunit
dc.subject Female
dc.subject Gene Expression Regulation, Neoplastic
dc.subject Humans
dc.subject Male
dc.subject Middle Aged
dc.subject Neoplasm Grading
dc.subject Receptors, Cell Surface
dc.subject Urinary Bladder Neoplasms
dc.title Detection of deleted in malignant brain tumors 1 and runt-related transcription factor 3 gene expressions in bladder carcinoma
dc.type Article
dc.relation.journal Molecular Biology Reports
dc.contributor.authorID 0000-0001-9399-0960
dc.identifier.volume 39
dc.identifier.issue 4
dc.identifier.startpage 4691
dc.identifier.endpage 4695
dc.relation.publicationCategory Uluslararası Hakemli Dergi
dc.identifier.index Scopus
dc.identifier.index WOS
dc.identifier.index PubMed

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