DSpace Repository

Investigation of siderophore, total matrix protease and elastase activity in Pseudomonas aeruginosa isolates from lower respiratory tract and extra-respiratory tract samples

Show simple item record

dc.contributor.author Demir, Melek
dc.contributor.author Cevahir, Nural
dc.contributor.author Kaleli, İlknur
dc.contributor.author Yildirim, U.
dc.contributor.author Şahin, R.
dc.contributor.author Çevik Tepeli, E.
dc.date.accessioned 2019-08-16T12:17:21Z
dc.date.available 2019-08-16T12:17:21Z
dc.date.issued 2008
dc.identifier.issn 0374-9096
dc.identifier.uri https://hdl.handle.net/11499/7184
dc.description.abstract Pseudomonas aeruginosa is an important opportunistic pathogen. P.aeruginosa strains secrete several virulence factors, in the form of extracellular proteins. Adhesins, pyocyanin, proteases, hemolysins, exotoxin A and exoenzyme S are some of the virulence factors found in P.aeruginosa strains. In this study, the presence of siderophore, total matrix protease and elastase activities were investigated in a total of 157 P.aeruginosa strains isolated from lower respiratory tract (n: 81; sputum, bronchoalveolar lavage, tracheal aspirate) and extrarespiratory sites (n: 76; urine, wound, blood) of hospitalized pati ents. Chrome azurol S (CAS) agar plates were used for detection of siderophore activity. Hide powder azure was used for the investigation of total matrix protease activity and elastin congo red was used to test elastase activity. All strains gave positive reaction on CAS agar. Enzyme activities of the test strains were compared with the activity of P.aeruginosa PAO1 positive control strain. Mean total matrix protease and elastase activities were less than P.aeruginosa PAO1 activity in the test strains, however, some strains exhibited activity higher than PAO1. There was no significant difference for mean protease and elastase activities between the strains isolated from lower respiratory tract samples and the others (p<0.05), as well as no difference with respect to antibiotic resistance (p<0.05). It was found that ceftazidime and cefoperazone were the most resistant agents in both groups (67.9% and 57.9% for ceftazidime and 49.3% and 48.7% for cefoperazone, respectively). It was concluded that further in vivo studies are necessary to clarify the role of virulence factors of P.aeruginosa in the establishment of infection in different body sites. en_US
dc.language.iso tr en_US
dc.relation.ispartof Mikrobiyoloji Bulteni en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.subject Elastase en_US
dc.subject Pseudomonas aeruginosa en_US
dc.subject Siderophore en_US
dc.subject Total matrix protease en_US
dc.subject Virulence factors en_US
dc.subject matrix metalloproteinase en_US
dc.subject pancreatic elastase en_US
dc.subject siderophore en_US
dc.subject virulence factor en_US
dc.subject antibiotic resistance en_US
dc.subject article en_US
dc.subject blood en_US
dc.subject drug effect en_US
dc.subject enzymology en_US
dc.subject human en_US
dc.subject injury en_US
dc.subject lung lavage en_US
dc.subject microbiology en_US
dc.subject pathogenicity en_US
dc.subject Pseudomonas infection en_US
dc.subject respiratory system en_US
dc.subject sputum en_US
dc.subject trachea en_US
dc.subject urine en_US
dc.subject Blood en_US
dc.subject Bronchoalveolar Lavage Fluid en_US
dc.subject Drug Resistance, Bacterial en_US
dc.subject Humans en_US
dc.subject Matrix Metalloproteinases en_US
dc.subject Pancreatic Elastase en_US
dc.subject Pseudomonas Infections en_US
dc.subject Respiratory System en_US
dc.subject Siderophores en_US
dc.subject Sputum en_US
dc.subject Trachea en_US
dc.subject Urine en_US
dc.subject Virulence Factors en_US
dc.subject Wounds and Injuries en_US
dc.title Investigation of siderophore, total matrix protease and elastase activity in Pseudomonas aeruginosa isolates from lower respiratory tract and extra-respiratory tract samples en_US
dc.type Article en_US
dc.identifier.volume 42 en_US
dc.identifier.issue 2 en_US
dc.identifier.startpage 197
dc.identifier.startpage 197 en_US
dc.identifier.endpage 208 en_US
dc.authorid 0000-0001-8764-7701
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.identifier.pmid 18697417 en_US
dc.identifier.wos WOS:000257024300001 en_US

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record