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Specific reactivity of mild/severe Alzheimer's disease patient's sera to antibody against Aβ1-40 epitope 17-21

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dc.contributor.author Bolukbasi Hatip, F.F.
dc.contributor.author Matsunaga, Y.
dc.contributor.author Yamada, T.
dc.date.accessioned 2019-08-16T12:16:37Z
dc.date.available 2019-08-16T12:16:37Z
dc.date.issued 2008
dc.identifier.issn 00016314 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/7148
dc.description.abstract Objectives - To detect the reactivity pattern of sera from patients with mild and severe Alzheimer's disease (AD) to specific antibodies targeting different epitopes in the primary structure of amyloid-beta (Aβ). Materials and methods - Sera from patients diagnosed with mild or severe AD were used. The reactivity of sera to monoclonal antibodies recognizing 1-7, 5-10, 9-14 and 17-21 epitopes of Aβ1-40 at 36-42°C was determined by an enzyme-linked immunosorbent assay. Proteinase K digestion of Aβ1-40 was investigated by dot blotting at 36 and 40°C. Results: - Sera of patients with AD displayed reactivity only with monoclonal antibody recognizing the epitope 17-21 (4G8). The reactivity of sera from patients with severe AD was less than that of sera from patients with mild AD at temperatures 36-41°C, with no difference at 42°C. Patients with severe AD displayed lesser digestion with proteinase K. Conclusions - Sera derived from patients with AD could react with monoclonal antibodies directed to 17-21 sequences of Aβ1-40 in a temperature-dependent manner. The severity of AD is associated with greater Aβ1-40 aggregation and resistance to proteinase K. The present results may be of value in staging and following up of patients with AD. Copyright © 2007 The Authors.
dc.language.iso English
dc.relation.isversionof 10.1111/j.1600-0404.2007.00959.x
dc.subject Aβ1-40
dc.subject Dot blot
dc.subject ELISA
dc.subject Monoclonal antibody 4G8 temperature
dc.subject Proteinase K
dc.subject Severe and mild Alzheimer's disease
dc.subject amyloid beta protein[1-40]
dc.subject epitope
dc.subject monoclonal antibody
dc.subject proteinase K
dc.subject adult
dc.subject aged
dc.subject Alzheimer disease
dc.subject antibody specificity
dc.subject article
dc.subject clinical article
dc.subject controlled study
dc.subject disease severity
dc.subject dot hybridization
dc.subject enzyme linked immunosorbent assay
dc.subject epitope mapping
dc.subject female
dc.subject gene sequence
dc.subject human
dc.subject immunoreactivity
dc.subject male
dc.subject protein aggregation
dc.subject protein structure
dc.subject serum
dc.subject temperature dependence
dc.subject Aged
dc.subject Aged, 80 and over
dc.subject Alzheimer Disease
dc.subject Amyloid beta-Protein
dc.subject Antibodies, Monoclonal
dc.subject Endopeptidase K
dc.subject Epitopes
dc.subject Female
dc.subject Humans
dc.subject Immune Sera
dc.subject Male
dc.subject Middle Aged
dc.subject Peptide Fragments
dc.title Specific reactivity of mild/severe Alzheimer's disease patient's sera to antibody against Aβ1-40 epitope 17-21
dc.type Article
dc.relation.journal Acta Neurologica Scandinavica
dc.identifier.volume 117
dc.identifier.issue 6
dc.identifier.startpage 404
dc.identifier.endpage 408
dc.identifier.index Scopus


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