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Ion-exchanger synthesis using reversible addition-fragmentation chain transfer polymerization

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dc.contributor.author Unsal, E.
dc.contributor.author Uguzdogan, E.
dc.contributor.author Patir, S.
dc.contributor.author Tuncel, A.
dc.date.accessioned 2019-08-16T12:10:19Z
dc.date.available 2019-08-16T12:10:19Z
dc.date.issued 2009
dc.identifier.issn 16159306 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/6742
dc.description.abstract An ion-exchanger with polyanionic molecular brushes was synthesized by a "grafting from" route based on "surface-controlled reversible addition-fragmentation chain transfer polymerization" (RAFT). The RAFT agent, PhC(S)SMgBr was covalently attached to monodisperse-porous poly(dihydroxypropyl methacrylate-co-ethylene dimethacrylate), poly(DHPM-co-EDM) particles 5.8 μm in size. The monomer, 3-sulfopropyl methacrylate (SPM), was grafted from the surface of poly(DHPM-co-EDM) particles with an immobilized chain transfer agent by the proposed RAFT protocol. The degree of polymerization of SPM (i. e. the molecular length of the polyanionic ligand) on the particles was controlled by varying the molar ratio of monomer/RAFT agent. The particles carrying polyanionic molecular brushes with different lengths were tested as packing material in the separation of proteins by ion exchange chromatography. The columns packed with the particles carrying relatively longer polyanionic ligands exhibited higher separation efficiency in the separation of four proteins. Plate heights between 130-200 μm were obtained. The ion-exchanger having poly-(SPM) ligand with lower degree of polymerization provided better peak-resolutions on applying a salt gradient with higher slope. The molecular length and the ion-exchanger group content of polyionic ligand were adjusted by controlling the degree of polymerization and the grafting density, respectively. This property allowed control of the separation performance of the ion-exchanger packing. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.language.iso English
dc.relation.isversionof 10.1002/jssc.200900003
dc.rights info:eu-repo/semantics/closedAccess
dc.subject HPLC
dc.subject Ion exchange chromatography
dc.subject Protein separation
dc.subject Reversible addition-fragmentation chain transfer polymerization
dc.subject Uniform porous particles
dc.subject Chromatographic analysis
dc.subject Chromatography
dc.subject Electric dipole moments
dc.subject Electric discharge machining
dc.subject Ethylene
dc.subject Grafting (chemical)
dc.subject High performance liquid chromatography
dc.subject Ion chromatography
dc.subject Ion exchangers
dc.subject Ions
dc.subject Ligands
dc.subject Micromachining
dc.subject Polymerization
dc.subject Polymers
dc.subject Proteins
dc.subject Separation
dc.subject Synthesis (chemical)
dc.subject Ion exchange
dc.subject 2,3 dihydroxypropylmethacrylate copolymer ethylenedimethacrylate
dc.subject 3 sulfupropyl methacrylate
dc.subject dihydroxypropyl methacrylate copolymer ethylene dimethacrylate
dc.subject monomer
dc.subject polymer
dc.subject unclassified drug
dc.subject analytic method
dc.subject article
dc.subject chemical reaction
dc.subject controlled study
dc.subject covalent bond
dc.subject flow rate
dc.subject ion exchange chromatography
dc.subject molecular weight
dc.subject particle size
dc.subject phase separation
dc.subject priority journal
dc.subject protein analysis
dc.subject protein polymerization
dc.subject reversible addition fragmentation chain transfer polymerization
dc.subject separation technique
dc.subject surface property
dc.subject synthesis
dc.subject Chromatography, Liquid
dc.subject Ion Exchange Resins
dc.subject Particle Size
dc.subject Polymethacrylic Acids
dc.subject Surface Properties
dc.subject Time Factors
dc.title Ion-exchanger synthesis using reversible addition-fragmentation chain transfer polymerization
dc.type Article
dc.relation.journal Journal of Separation Science
dc.contributor.authorID 0000-0003-0607-1304
dc.identifier.volume 32
dc.identifier.issue 11
dc.identifier.startpage 1791
dc.identifier.endpage 1800
dc.relation.publicationCategory Uluslararası Hakemli Dergi
dc.identifier.index Scopus
dc.identifier.index WOS


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