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Naringenin inhibits neointimal hyperplasia following arterial reconstruction with interpositional vein graft

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dc.contributor.author Cayci, C.
dc.contributor.author Wahlquist, T.C.
dc.contributor.author Seckin, S.I.
dc.contributor.author Ozcan, V.
dc.contributor.author Tekinay, A.B.
dc.contributor.author Martens, T.P.
dc.contributor.author Oz, M.C.
dc.contributor.author Ascherman, J.A.
dc.date.accessioned 2019-08-16T12:08:46Z
dc.date.available 2019-08-16T12:08:46Z
dc.date.issued 2010
dc.identifier.issn 01487043 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/6578
dc.description.abstract Vessels respond to injury by a healing process that includes the development of neointima. Stenosis secondary to neointima formation is the main cause of failure following arterial reconstructions. Vessel wall homeostasis is regulated by proinflammatory cytokines that affect smooth muscle cell proliferation, growth, migration, and death. We assessed the hypothesis that naringenin, a flavinoid possessing anti-inflammatory, antioxidant, and antiproliferative activities, reduces neointimal hyperplasia (NIH) following vascular injury.Arterial injury was created by interposition grafting of autologous right superficial epigastric vein graft into the right femoral artery (FA) in 48 male Sprague-Dawley rats. Following injury, the rats were divided into 4 groups (n = 12). Two groups were treated with naringenin (100 mg/kg intraperitoneal q daily) for 2 and 4 weeks each while 2 control groups received normal saline for the same durations. For Sham group (n = 10), the FA and vein were isolated without any additional procedure. Rats were killed at the end of treatment regimen in all groups, and FAs were harvested. Thickness of intima was measured in histologic sections, and levels of platelet derived growth factor (PDGF)-BB, TNFα, and Ki67 labeling index (Ki67 LI) were quantified in immunohistochemical analyses to assess the amount of NIH and mechanisms underlying its formation.Although there was no significant difference between the groups at 2 weeks, neointima thickness was lower in the naringenin treated group at 4 weeks (23.7 ± 2.3 vs. 35.6 ± 2.6 μm in control group; P < 0.001). The levels of PDGF-BB, and TNFα were lower in naringenin treated groups at both 2 weeks (PDGF-BB [0.21% ± 0.03% versus 0.39% ± 0.05% in control group, P < 0.001), TNFα (21.2% ± 0.8% vs. 36.1% ± 1.9% in control group, P < 0.001]) and 4 weeks (PDGF-BB [0.25% ± 0.03% vs. 0.57% ± 0.09% in control group, P < 0.001], TNFα [25.5% ± 1.8% vs. 45.0% ± 2.9% in control group, P < 0.001]). Ki67 LI was lower in naringenin treated groups at 2 weeks (13.9% ± 2.8% vs. 18.7% ± 3.7% in control group, P < 0.05), and at 4 weeks (17.5% ± 2.6% vs. 31.1% ± 4.7% in control group, P < 0.001), indicating a lower level of cellular proliferation.Naringenin reduces NIH following arterial reconstruction. This may be mediated by a decrease in PDGF-BB and TNFα levels and the resulting down-regulation of smooth muscle cells' migration and proliferation. Copyright © 2009 by Lippincott Williams & Wilkins.
dc.language.iso English
dc.relation.isversionof 10.1097/SAP.0b013e31819b03cd
dc.subject Arterial reconstruction with interpositional vein graft
dc.subject Naringenin
dc.subject Neointimal hyperplasia
dc.subject antioxidant
dc.subject flavanone derivative
dc.subject naringenin
dc.subject animal
dc.subject article
dc.subject autotransplantation
dc.subject drug administration
dc.subject drug effect
dc.subject femoral artery
dc.subject hyperplasia
dc.subject immunohistochemistry
dc.subject intima
dc.subject male
dc.subject methodology
dc.subject pathology
dc.subject plastic surgery
dc.subject postoperative care
dc.subject rat
dc.subject Sprague Dawley rat
dc.subject transplantation
dc.subject vein
dc.subject Animals
dc.subject Antioxidants
dc.subject Drug Administration Schedule
dc.subject Femoral Artery
dc.subject Flavanones
dc.subject Hyperplasia
dc.subject Immunohistochemistry
dc.subject Male
dc.subject Postoperative Care
dc.subject Rats
dc.subject Rats, Sprague-Dawley
dc.subject Reconstructive Surgical Procedures
dc.subject Transplantation, Autologous
dc.subject Tunica Intima
dc.subject Veins
dc.title Naringenin inhibits neointimal hyperplasia following arterial reconstruction with interpositional vein graft
dc.type Article
dc.relation.journal Annals of Plastic Surgery
dc.identifier.volume 64
dc.identifier.issue 1
dc.identifier.startpage 105
dc.identifier.endpage 113
dc.identifier.index Scopus

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