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Characterization of drug metabolizing enzymes and assessment of aging in the gilthead seabream (Sparus aurata) liver

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dc.contributor.author Akdoğan, Hatice Ardağ
dc.contributor.author Şen, Alaattin
dc.date.accessioned 2019-08-16T12:08:00Z
dc.date.available 2019-08-16T12:08:00Z
dc.date.issued 2010
dc.identifier.issn 03758427 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/6502
dc.description.abstract Organic anthropogenic compounds such as polycyclic aromatic hydrocarbons, polychlorinated biphenyls, nitroaromatics, dioxins, various pesticides and natural compounds can enter the aquatic environment. These lipophilic compounds are readily taken up into the tissues of aquatic organisms where biotransformation via Phase I and Phase II metabolism can in part, determine the fate and toxicity of the xenobiotics. In this study on gilthead seabream (Sparus aurata), liver microsomal ethoxyresorufin O-deethylase (EROD), methoxyresorufin O-demethylase (MROD), penthyloxyresorufin O-depenthylase (PROD), benzyloxyresorufin O-debenzylase (BROD), aniline 4-hydroxylase (A4H), N-nitrosodimethylamine N-demethylase (NDMA-DE), aminopyrine N-demethylase (APND), caffeine N-demethylase (CN3D) and erythromycin N-demethylase (ERND) were determined. A4H, ERND, NDMA-ND, EROD and PROD activities increased in gilthead seabream (Sparus aurata) liver microsomes of different ages, while CN3D and APND activities decreased. MROD activities were barely detectable in mature fish whereas BROD activities were not detectable at all ages studied. Results were also recorded at the protein level by Western blotting using anti-CYP1A, CYP2E and CYP3A antibodies. In conclusion, elevated levels of gilthead seabream liver microsomal CYP450 enzyme activities might reveal possible exposure to various exogenous compounds, which might affect the desired responses to drugs, hormones and dietary supplements used during breeding. © 2007 Veterinarni Medicina.
dc.language.iso English
dc.publisher Czech Academy of Agricultural Sciences
dc.relation.isversionof 10.17221/2982-VETMED
dc.rights info:eu-repo/semantics/openAccess
dc.subject Drug metabolizing enzymes
dc.subject Fish
dc.subject Gilthead seabream
dc.subject Sparus aurata
dc.title Characterization of drug metabolizing enzymes and assessment of aging in the gilthead seabream (Sparus aurata) liver
dc.type Article
dc.relation.journal Veterinarni Medicina
dc.contributor.authorID 0000-0002-8444-376
dc.identifier.volume 55
dc.identifier.issue 9
dc.identifier.startpage 463
dc.identifier.endpage 471
dc.relation.publicationCategory Uluslararası Hakemli Dergi
dc.identifier.index Scopus
dc.identifier.index WOS


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