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Dose dependent effect of C-type natriuretic peptide signaling in glycosaminoglycan synthesis during TGF-β1 induced chondrogenic differentiation of mesenchymal stem cells

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dc.contributor.author Tezcan, B.
dc.contributor.author Serter, Sema
dc.contributor.author Kiter, Esat
dc.contributor.author Tufan, A.C.
dc.date.accessioned 2019-08-16T12:05:41Z
dc.date.available 2019-08-16T12:05:41Z
dc.date.issued 2010
dc.identifier.issn 15672379 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/6294
dc.description.abstract Recent investigations credited important roles to C-type natriuretic peptide (CNP) signaling during chondrogenesis. This study investigated the putative role of CNP in transforming growth factor (TGF)-β1 induced in vitro chondrogenic differentiation of mesenchymal stem cells (MSCs) in pellet culture. MSCs were derived from human trabecular bone and were characterized on the basis of their cell surface antigens and adipogenic, osteogenic, and chondrogenic differentiation potential. TGF-β1 induced chondrogenic differentiation and glycosaminoglycan (GAG) synthesis was analyzed on the basis of basic histology, collagen type II, Sox 9 and aggrecan expressions, and Alcian blue staining. Results revealed that human trabecular bone-derived MSCs express CNP and NPR-B analyzed on the basis of RT-PCR and immunohistochemistry. In pellet cultures of MSCs TGF-β1 successfully induced chondrogenic differentiation and GAG synthesis. RT-PCR analyses of both CNP and NPR-B during this process revealed an activation of this signaling pathway in response to TGF-β1. Similar cultures induced with TGF-β1 and treated with different doses of CNP showed that CNP supplementation at 10-8 and 10-7 M concentrations significantly increased GAG synthesis in a dose dependent manner, whereas at 10-6 M concentration this stimulatory effect was diminished. In conclusion, CNP/NPR-B signaling pathway is activated during TGF-β1 induced chondrogenic differentiation of human trabecular bone-derived MSCs and may strongly be involved in GAG synthesis during this process. This effect is likely to be a dose-dependent effect. © 2010 Springer Science+Business Media B.V.
dc.language.iso English
dc.relation.isversionof 10.1007/s10735-010-9284-4
dc.rights info:eu-repo/semantics/closedAccess
dc.subject Chondrogenesis
dc.subject CNP
dc.subject GAG synthesis
dc.subject NPR-B
dc.subject TGF-β
dc.subject aggrecan
dc.subject alcian blue
dc.subject collagen type 2
dc.subject membrane antigen
dc.subject natriuretic peptide receptor B
dc.subject natriuretic peptide type C
dc.subject transcription factor Sox9
dc.subject transforming growth factor beta1
dc.subject adipogenesis
dc.subject adult
dc.subject aged
dc.subject article
dc.subject bone development
dc.subject cell assay
dc.subject cell differentiation
dc.subject cell membrane potential
dc.subject cell migration
dc.subject chondrogenesis
dc.subject concentration response
dc.subject controlled study
dc.subject glycosaminoglycan metabolism
dc.subject human
dc.subject human cell
dc.subject human tissue
dc.subject immunohistochemistry
dc.subject in vitro study
dc.subject mesenchymal stem cell
dc.subject nucleotide sequence
dc.subject priority journal
dc.subject protein analysis
dc.subject protein expression
dc.subject protein function
dc.subject reverse transcription polymerase chain reaction
dc.subject signal transduction
dc.subject trabecular bone
dc.subject Aged
dc.subject Antigens, Surface
dc.subject Bone and Bones
dc.subject Cell Differentiation
dc.subject Cell Separation
dc.subject Glycosaminoglycans
dc.subject Humans
dc.subject Immunohistochemistry
dc.subject Mesenchymal Stem Cells
dc.subject Middle Aged
dc.subject Natriuretic Peptide, Brain
dc.subject Natriuretic Peptide, C-Type
dc.subject Reverse Transcriptase Polymerase Chain Reaction
dc.subject Signal Transduction
dc.subject Transforming Growth Factor beta1
dc.title Dose dependent effect of C-type natriuretic peptide signaling in glycosaminoglycan synthesis during TGF-β1 induced chondrogenic differentiation of mesenchymal stem cells
dc.type Article
dc.relation.journal Journal of Molecular Histology
dc.identifier.volume 41
dc.identifier.issue 4-5
dc.identifier.startpage 247
dc.identifier.endpage 258
dc.relation.publicationCategory Uluslararası Hakemli Dergi
dc.identifier.index Scopus
dc.identifier.index WOS


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