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Effect of amifostine pre-treatment against adriamycin-induced lipid peroxidation in rat heart

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dc.contributor.author Bolaman, Z.
dc.contributor.author Akalin, N.
dc.contributor.author Koseoglu, M.H.
dc.contributor.author Demir, S.
dc.contributor.author Kadikoylu, G.
dc.contributor.author Atalay, H.
dc.contributor.author Aslan, D.
dc.date.accessioned 2019-08-16T11:44:39Z
dc.date.available 2019-08-16T11:44:39Z
dc.date.issued 2003
dc.identifier.issn 1108-2682
dc.identifier.uri https://hdl.handle.net/11499/5317
dc.description.abstract To investigate the effect of amifostine (AMI, WR-2721) against lipid peroxidation caused by adriamycin in rat heart tissue, male Wistar rats, weighing 190-220 g, were divided into three groups and treated as follows: 1) vehicle (saline) control (n = 6); 2) Adriamycin (10 mg/kg, intraperitoneally) (n = 6); 3) Adriamycin plus amifostine (200 mg/kg) (n = 6). Amifostine was applicated to rats 30 minutes previously from Adriamycin application. Rats were sacrificed on third day after drug application and hearts were removed. The hearts were washed with cold saline solution and were stored at -20°C until analysed. The tissues were thawed and homogenised with an Ultra Turrax. Lipid peroxidation was assayed as the malondialdehyde (MDA) levels reacting with thiobarbituric acid, according to the method of Ohkawa. Tissue MDA levels were significantly increased by adriamycin (p<0.05). MDA levels were 168±15 nmol/g wt in the control group. Adriamycin increased the MDA levels to 212±34 nmol/g wt. In the adriamycin plus AMI group, the heart tissue MDA levels were not different from the control levels (156±31 nmol/g wt). This study showed that the MDA levels were not increased if AMI were given previously Adriamycin in rats. AMI can reduce adriamycin induced cardiotoxicity and may be protective for adriamycin induced free radical damage in the heart tissues. So we suggest that the protective effect of AMI pretreatment on ADR heart toxicity should be evaluated extensively. en_US
dc.language.iso en en_US
dc.relation.ispartof HAEMA en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.subject Adriamycine en_US
dc.subject Amifostine en_US
dc.subject Cardiotoxicity en_US
dc.subject Lipidperoxidation en_US
dc.subject amifostine en_US
dc.subject doxorubicin en_US
dc.subject free radical en_US
dc.subject malonaldehyde en_US
dc.subject thiobarbituric acid en_US
dc.subject animal experiment en_US
dc.subject animal tissue en_US
dc.subject article en_US
dc.subject cardiotoxicity en_US
dc.subject controlled study en_US
dc.subject drug effect en_US
dc.subject drug mechanism en_US
dc.subject enzyme activity en_US
dc.subject heart protection en_US
dc.subject histopathology en_US
dc.subject lipid peroxidation en_US
dc.subject male en_US
dc.subject nonhuman en_US
dc.subject rat en_US
dc.title Effect of amifostine pre-treatment against adriamycin-induced lipid peroxidation in rat heart en_US
dc.type Article en_US
dc.identifier.volume 6 en_US
dc.identifier.issue 1 en_US
dc.identifier.startpage 61
dc.identifier.startpage 61 en_US
dc.identifier.endpage 64 en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US

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