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Determination of Teucrium chamaedrys volatiles by using direct thermal desorption-comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry

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dc.contributor.author Özel, M.Z.
dc.contributor.author Göǧüş, F.
dc.contributor.author Lewis, A.C.
dc.date.accessioned 2019-08-16T11:35:18Z
dc.date.available 2019-08-16T11:35:18Z
dc.date.issued 2006
dc.identifier.issn 00219673 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/4594
dc.description.abstract The direct qualification and quantification of the volatile components of Teucrium chamaedrys was studied using a direct thermal desorption (DTD) technique with comprehensive two-dimensional (2D) gas chromatography-time-of-flight mass spectrometry (GC × GC-TOF/MS). The GC × GC separation chromatographically resolved hundreds of components within this sample, and with the separation coupled with TOF/MS for detection, high probability identifications were made for 68 compounds. The quantitative results were determined through the use of internal standards and the desorption of differing amounts of raw material in the injector. The highest yield of volatile compounds (0.39%, w/w) was obtained at 150 °C thermal desorption temperature using 1.0 mg of dried sample placed in a glass injector liner when studied over the range 1.0-7.0 mg. Lowest yield of 0.33% (w/w) was found for the largest sample size of 7.0 mg. Relative standard deviation (RSD) for 10 replicates at each size sample were in the range 3.9-21.6%. The major compounds identified were β-pinene, germacrene D, α-pinene, α-farnesene, α-gurjunene, γ-elemene and γ-cadinene. All identified compounds were quantified using total ion chromatogram (TIC) peak areas. DTD is a promising method for quantitative analysis of complex mixtures, and in particular for quantitative analysis of plant samples, which can yield data without the traditional obligation for costly and time-consuming extraction techniques. © 2006 Elsevier B.V. All rights reserved.
dc.language.iso English
dc.relation.isversionof 10.1016/j.chroma.2006.02.036
dc.subject Direct thermal desorption
dc.subject Gas chromatography
dc.subject Teucrium chamaedrys
dc.subject Time-of-flight mass spectrometry
dc.subject Volatile organic compounds
dc.subject Desorption
dc.subject Mass spectrometry
dc.subject Probability
dc.subject Raw materials
dc.subject Separation
dc.subject Statistical methods
dc.subject Total ion chromatogram (TIC)
dc.subject plant extract
dc.subject Teucrium chamaedrys extract
dc.subject unclassified drug
dc.subject volatile agent
dc.subject analytic method
dc.subject article
dc.subject calibration
dc.subject direct thermal desorption
dc.subject drug isolation
dc.subject gas chromatography
dc.subject germander
dc.subject particle size
dc.subject priority journal
dc.subject probability
dc.subject qualitative analysis
dc.subject quantitative analysis
dc.subject separation technique
dc.subject time of flight mass spectrometry
dc.subject Gas Chromatography-Mass Spectrometry
dc.subject Teucrium
dc.subject Volatilization
dc.title Determination of Teucrium chamaedrys volatiles by using direct thermal desorption-comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry
dc.type Article
dc.relation.journal Journal of Chromatography A
dc.identifier.volume 1114
dc.identifier.issue 1
dc.identifier.startpage 164
dc.identifier.endpage 169
dc.identifier.index Scopus

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