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Apoptotic cell death and its relationship to gastric carcinogenesis

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dc.contributor.author Bir, Ferda
dc.contributor.author Çallı-Demirkan, Neşe
dc.contributor.author Tufan, A. Çevik
dc.contributor.author Akbulut, Metin
dc.contributor.author Satiroglu-Tufan, N. Lale
dc.date.accessioned 2019-08-16T11:32:56Z
dc.date.available 2019-08-16T11:32:56Z
dc.date.issued 2007
dc.identifier.issn 10079327 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/4229
dc.description.abstract Aim: To investigate the apoptotic process of cells within the intestinal metaplasia areas co-localizing with chronic gastritis and gastric carcinomas and to analyze the involvement of proteins regulating apoptosis in the process of intestinal metaplasia related gastric carcinogenesis. Methods: Forty-two gastric carcinoma and seventeen chronic gastritis cases were included in this study. All cases were examined for the existence of intestinal metaplasia. Ten cases randomly selected from each group were processed for TUNEL assay. TUNEL positive cells within the intestinal metaplasia areas, colocalizing either to gastric carcinoma or chronic gastritis, were counted and converted to apoptotic indices. In addition, p53, bcl-2 and bax expression patterns within these tissues were analyzed on the basis of immunohistochemistry. Results: Twenty-eight of the cases were intestinal and 14 of the cases were diffuse type adenocarcinomas. 64% (27/42) of the gastric carcinoma cases had intestinal metaplasia. Intestinal metaplasia co-localized more with intestinal type carcinomas compared with diffuse type carcinomas [75% (21/28) vs 42% (6/14), respectively; P ≤ 0.05]. The mean apoptotic index in tumor cells was 0.70 ± 0.08. The mean apoptotic index in intestinal metaplasias co-localizing to tumors was significantly higher than that of intestinal metaplasias co-localizing to chronic gastritis (0.70 ± 0.03 vs 0.09 ± 0.01, respectively; P ≤ 0.05). p53 positivity was not observed in areas of intestinal metaplasia adjacent to tumors or chronic gastritis. Intestinal metaplasia areas adjacent to tumors showed lower cytoplasmic bcl-2 positivity compared to intestinal metaplasia areas adjacent to chronic gastritis [55.5% (15/27) vs 70.5% (12/17), respectively]. On the other hand, intestinal metaplasia areas adjacent to tumors showed significantly higher cytoplasmic bax positivity compared to intestinal metaplasia areas adjacent to chronic gastritis [44.4% (12/27) vs 11.7% (2/17), respectively; P ≤ 0.05]. Conclusion: Existence of apoptotic cells on the basis of TUNEL positivity is shown in intestinal metaplasias co-localizing to both diffuse and intestinal type gastric cancers in this study. Our results also suggested bax expression dependent induction of apoptosis especially in intestinal metaplasia areas adjacent to tumors. These findings strongly support the involvement of apoptotic mechanisms in the process of gastric carcinogenesis especially in the transition from intestinal metaplasia to gastric cancer. It may be suggested that induction of apoptosis in intestinal metaplasia areas adjacent to tumors may involve different mechanisms than induction by chronic inflammation. © 2007 The WJG Press. All rights reserved.
dc.language.iso English
dc.publisher WJG Press
dc.relation.isversionof 10.3748/wjg.v13.i23.3183
dc.rights info:eu-repo/semantics/openAccess
dc.subject Apoptosis
dc.subject Bax
dc.subject Bcl-2
dc.subject Gastric cancer
dc.subject Intestinal metaplasia
dc.subject p53
dc.subject TUNEL staining
dc.subject protein Bax
dc.subject protein bcl 2
dc.subject protein p53
dc.subject adult
dc.subject aged
dc.subject apoptosis
dc.subject article
dc.subject cancer classification
dc.subject chronic gastritis
dc.subject controlled study
dc.subject cytoplasm
dc.subject female
dc.subject gastritis
dc.subject histopathology
dc.subject human
dc.subject human tissue
dc.subject immunohistochemistry
dc.subject intestine metaplasia
dc.subject major clinical study
dc.subject male
dc.subject malignant transformation
dc.subject nick end labeling
dc.subject protein analysis
dc.subject protein expression
dc.subject protein function
dc.subject regulatory mechanism
dc.subject stomach adenocarcinoma
dc.subject stomach carcinogenesis
dc.subject stomach carcinoma
dc.subject tissue distribution
dc.title Apoptotic cell death and its relationship to gastric carcinogenesis
dc.type Article
dc.relation.journal World Journal of Gastroenterology
dc.contributor.authorID 0000-0001-5860-100X
dc.contributor.authorID 0000-0002-5920-0475
dc.contributor.authorID 0000-0001-9399-0960
dc.identifier.volume 13
dc.identifier.issue 23
dc.identifier.startpage 3183
dc.identifier.endpage 3188
dc.relation.publicationCategory Uluslararası Hakemli Dergi
dc.identifier.index Scopus
dc.identifier.index WOS
dc.identifier.index PubMed


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