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Orientation of photosystem i on graphene through cytochrome: C 553 leads to improvement in photocurrent generation

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dc.contributor.author Kiliszek, M.
dc.contributor.author Harputlu, E.
dc.contributor.author Szalkowski, M.
dc.contributor.author Kowalska, D.
dc.contributor.author Unlu, Cumhur Gökhan
dc.contributor.author Haniewicz, P.
dc.contributor.author Abram, M.
dc.contributor.author Wiwatowski, K.
dc.contributor.author Niedziółka-Jönsson, J.
dc.contributor.author MaćKowski, S.
dc.contributor.author Ocakoglu, K.
dc.contributor.author Kargul, J.
dc.date.accessioned 2019-08-16T13:34:36Z
dc.date.available 2019-08-16T13:34:36Z
dc.date.issued 2018
dc.identifier.issn 20507488 (ISSN)
dc.identifier.uri http://acikerisim.pau.edu.tr:8080/xmlui/handle/11499/11033
dc.description.abstract We report the fabrication of an oriented bioelectrode of photosystem I (PSI) on single-layer graphene (SLG). This bioelectrode demonstrates improved photocurrent generation, which can be directly attributed to the molecular conductive interface formed by cytochrome c553 (cyt c553) promoting the uniform orientation of PSI with its donor side towards the electrode. The conductive interface between PSI-cyt c553 and SLG is facilitated by a monolayer composed of π-π-stacked pyrene functionalized with the Ni-NTA moiety, which binds the His6-tagged cyt c553. The surface uniformity of the PSI protein orientation in the electrode structure is evidenced by cross-sectional scanning electron microscopy and fluorescence microscopy, with the latter also proving the efficient electronic coupling between majority of the PSI complexes and graphene. With the uniform organization of the biological photoactive layer, photocurrents are generated at the open circuit potential, which can be further increased when a negative potential is applied. Indeed, at the highest applied negative potential (-0.3 V), over 5-fold increase in the cathodic photocurrent for the PSI complexes conjugated via cyt c553 to the SLG substrate is observed compared with that obtained for the randomly oriented structure where PSI is physisorbed on graphene. These results indicate the key role of a strictly defined orientation of photoactive proteins on electrodes for proper electron transfer and substantial improvement in photocurrent generation in the present or similar bioelectrode architectures. © 2018 The Royal Society of Chemistry.
dc.language.iso English
dc.publisher Royal Society of Chemistry
dc.relation.isversionof 10.1039/c8ta02420k
dc.rights info:eu-repo/semantics/embargoedAccess
dc.title Orientation of photosystem i on graphene through cytochrome: C 553 leads to improvement in photocurrent generation
dc.type Article
dc.relation.journal Journal of Materials Chemistry A
dc.identifier.volume 6
dc.identifier.issue 38
dc.identifier.startpage 18615
dc.identifier.endpage 18626
dc.relation.publicationCategory Uluslararası Hakemli Dergi
dc.identifier.index Scopus
dc.identifier.index WOS


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